Quick Details

Product name:Methacrylated Alginate;ALMA

Product category:Blu-ray curing and modified gelatin based series

Appearance:white powder

Forming and curing process:temperature sensitive and blue light curing

Applicable cells:chondrocytes, osteoblasts, cardiomyocytes, skin cells, nerve cells, fibroblasts, liver cells, kidney cells, bladder transitional epithelial cells, limbal stem cells, etc.

Print tissue:Vascular tissue, bone, cartilage, etc.

Adapter printer:extruded bio-printer such as EnvisionTec in Germany and Allevi (formerly BioBots) in the US

Sterile grade:sterile, no mycoplasma, no endotoxin

Quality level:Bioink-qualified

Description

ALMA is a photosensitive biomaterial, it is used in combination with blue or ultraviolet light initiators to crosslink and cure under the action of blue light or ultraviolet light, the higher the concentration of ALMA, the greater the hardness after curing, it has good biocompatibility and can be mixed with cells for bio 3D printing.

Feature

ALMA has low cost, biostable, fast ion crosslinking ability, and suitable mechanical properties, but slow degradation, low cell adhesion, and need to be mixed with other biological materials.

Quality control test

Each batch of ALMA was confirmed by H NMR, HPLC and cytology, and its chemical structure, MA grafting rate was 20-30%, no cytotoxicity; ALMA formulated hydrogel, after adding blue initiator LAP, It can be cured quickly under the action of blue light.

Figure 1: Printed ear cartilage

Figure 2: Chondrocytes in ALMA hydrogel

Instructions

1.Weigh the required amount of ALMA for printing, acetone sterilization

2.Prepare 0.25~0.5% LAP solution, dissolve at 37℃, filter and sterilize with 0.22μm filter;

3.Under sterile conditions, add ALMA, the final concentration is 2~3%, Dissolve overnight;

4.Mixed with cells, placed in the cartridge of the Blu-ray bio 3D printer;

5.3D printing, blue light (wavelength 405 nm) curing.

Packaging

As your requirements

Storage

Store in -20℃, dry, well ventilated area, away from direct sunlight.

Shelf life

1 year