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Product name:GelMA;Methacrylated Gelatin
Product category:Blu-ray curing and modified gelatin based series
Forming and curing process:temperature sensitive and blue light curing
Applicable cells:chondrocytes, osteoblasts, cardiomyocytes, skin cells, nerve cells, fibroblasts, liver cells, kidney cells, bladder transitional epithelial cells, limbal stem cells, etc.
Print tissue:bone, cartilage, heart and blood vessels, etc.
Adapter printer:extruded bio-printer such as EnvisionTec in Germany and Allevi (formerly BioBots) in the US
Sterile grade:sterile, no mycoplasma, no endotoxin
GelMA is a photosensitive biomaterial, it is used in combination with blue or ultraviolet light initiators to crosslink and cure under the action of blue light or ultraviolet light, the higher the concentration of GelMA, the greater the hardness after curing, it has good biocompatibility and can be mixed with cells for bio 3D printing.
GelMA hydrogels are very similar to the basic properties of natural extracellular matrices (ECM), which facilitate cell adhesion and proliferation. Moreover, its high moisture content, small molecules are easy to penetrate, which is beneficial to nutrient exchange, so it is suitable for encapsulating cells in three-dimensional culture.
Quality control test
Each batch of GelMA was confirmed by H NMR, HPLC and cytology, and its chemical structure, MA grafting rate was 50-60%, no cytotoxicity; GelMA formulated hydrogel, after adding blue initiator LAP, It can be cured quickly under the action of blue light.
Figure 1: GelMA hydrogel cured under blue light
Figure 2: Chondrocytes in GelMA hydrogels
1.Prepare 0.25~0.5% LAP solution, dissolve at 37℃;
2.Add GelMA, the final concentration is 5~10%, dissolve at 60℃, filter and sterilize with 0.22μm filter;
3.Mixed with cells, placed in the cartridge of the Blu-ray bio 3D printer;
4.3D printing, blue light (wavelength 405 nm) curing.
Attention:When the room temperature is below 25℃, the GelMA solution will solidify and need to be heated and melted before use.
As your requirements
Store in -20℃, dry, well ventilated area, away from direct sunlight.