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Product name:Chitosan-Methacrylic Acid-Lactic Acid;Chi-MA-LA
Product category:Blu-ray curing and modified gelatin based series
Forming and curing process:temperature sensitive and blue light curing
Applicable cells:chondrocytes, osteoblasts, cardiomyocytes, skin cells, nerve cells, fibroblasts, liver cells, kidney cells, bladder transitional epithelial cells, limbal stem cells, etc.
Print tissue:Bone, cartilage, etc.
Adapter printer:extruded bio-printer such as EnvisionTec in Germany and Allevi (formerly BioBots) in the US
Sterile grade:sterile, no mycoplasma, no endotoxin
Chi-MA-LA is a photosensitive biomaterial, it is used in combination with blue or ultraviolet light initiators to crosslink and cure under the action of blue light or ultraviolet light, the higher the concentration of Chi-MA-LA, the greater the hardness after curing, it has good biocompatibility and can be mixed with cells for bio 3D printing.
Quality control test
Each batch of Chi-MA-LA was confirmed by H NMR, HPLC and cytology, and its chemical structure, MA grafting rate was 20-30%, no cytotoxicity; Chi-MA-LA formulated hydrogel, after adding blue initiator LAP, It can be cured quickly under the action of blue light.
1.Weigh the required amount of Chi-MA-LA for printing, acetone sterilization
2.Prepare 0.25~0.5% LAP solution, dissolve at 37℃, filter and sterilize with 0.22μm filter;
3.Under sterile conditions, add Chi-MA-LA, the final concentration is 1~2%, Dissolve overnight;
4.Mixed with cells, placed in the cartridge of the Blu-ray bio 3D printer;
5.3D printing, blue light (wavelength 405 nm) curing.
As your requirements
Store in -20℃, dry, well ventilated area, away from direct sunlight.