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3-Aminophthalic acid hydrazide;
White to yellowish powder
Luminol powder is easily soluble in lye, soluble in dilute acid, almost insoluble in water, and hardly soluble in alcohol. When the neutral or light acid solution is exposed to ultraviolet light, it shows strong bright blue fluorescence.
Luminol is one of the oldest and most commonly used reagents. It can be oxidized by peroxides under alkaline conditions and converted into excited aminophthalic acid, which emits strong fluorescence. The oxidation-reduction reaction between luminol and peroxide requires a catalyst. This catalyst is generally a multivalent metal ion, peroxidase such as iron, horseradish peroxidase, etc. This method is often used to detect peroxides , The content of heavy metals, peroxidase, and the derived free radical detection, toxicant analysis and analysis methods based on peroxidase and glucose oxidase.
Luminol and hydrogen peroxide
For example, the glucose oxidase/catalase probe can detect the concentration of hydrogen peroxide or glucose in the sample, and the response time is only 0.5s (dynamic method). Combining chemiluminescent substances with immunological reactions and using light reactions to express the concentration of the tested immune components is called chemiluminescence immunoassay technology (CLIA).
In 1976, Shmeder proposed chemiluminescence immunoassay, using luminol-hydrogen peroxide and its derivative ABEI as the detection reaction display system. At present, luminol and its derivatives luminol sodium salt are the most commonly used markers in chemiluminescence immunoassay applications. Under alkaline conditions, they can release a large number of photons through the catalysis of microperoxidase. Luminol can be used for WesternBlot of HRP-labeled antibodies and nucleic acid hybridization detection of HRP-labeled probes, and it is also used in modern criminal investigation bloodstains. Detection.
What is luminol used for
Luminol is often used as a chemiluminescence reagent and indicator, and is often used for chemiluminescence analysis, such as metal cations, blood immunity and so on.
Luminol is a strong acid that can irritate the eyes, skin and respiratory tract.
How to make luminol
Add 3-nitrophthalic acid to 8% hydrazine aqueous solution and heat to melt the solid. Add ethylene glycol, evaporate the excess water in the solution in the evaporator, heat at 215-220℃ for 2 minutes to close the heterocyclic ring, and obtain a yellow nitro compound after cooling. Cool, add 10% sodium hydroxide solution, stir, add sodium dithionate, heat to boiling, keep for 5 minutes, add acetic acid, and cool to get a bright yellow product. Let it stand overnight and filter to obtain luminol.
Where to buy luminol
Taian Green Industry Co., Ltd is a professional luminol manufacturer and supplier in China. We have decades of production experience and provide reliable quality products for the majority of scientific research institutions and universities.
Store in a dry, well-ventilated place away from direct sunlight.