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DL-Dithiothreitol, referred to as DTT, is a small molecule organic reducing agent. DL-Dithiothreitol is mainly used in the field of biotechnology as a protein lysis reagent. DTT is a strong reducing agent, and its reducibility is largely due to the conformational stability of the six-membered ring (containing disulfide bonds) in its oxidation state. One of the uses of DTT is as a reducing agent and deprotecting agent for sulfhydryl DNA.
Dithiothreitol has an antioxidant effect, it can protect the reducing group on the enzyme molecule, maintain a reducing environment, and stabilize the activity of the enzyme. DTT also contains certain RNase inhibitors, which can inhibit RNase activity. DL-Dithiothreitol has a similar effect to mercaptoethanol (dimercaptoethanol), but DTT has a much smaller pungent odor and is much less toxic than mercaptoethanol.
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Store in a dry, well-ventilated place away from direct sunlight.